Atawia et al. 2016 (Article) Ananas

Studies on Micropropagation of Pineapple (Ananas comosus L.)

Author(s):—A.R. Atawia, F.A. El L., S.F. El G., S.S. Sherif & O.M. Kotb

Corresponding email:—osama_kotb@yahoo.com

Publication:—Middle East Journal of Agriculture Research 5(2): 224-232. (2016)

Abstract:—The present study was conducted at the Tissue Culture Laboratory, Horticulture, Research Institute, Agricultural Research Center (ARC), Egypt during the period from December 2013 to March 2016 to investigate the effect of different type Murashige and Skooge (MS), Gamborge (B5) and Woody plant media (WPM) at four salt concentrations (Full, ¾, ½ and ¼) of culture media on micropropagability of pineapple (Ananas comosus L. var. Smooth Cayenne) during establishment stage. Shootlet proliferations were investigated at different concentrations of 6-benzyle amino purine (BAP) and kinitine (Kin) at 0.25, 0.5, 1.0 and 2.0 mg/l for each, during two successive subcultures. Finally, rooting capacity were studied by various concentrations of indole butyric acid (IBA) and indole acetic acid (IAA) at1.0, 2.0 and 3.0 mg/l on media containing activated charcoal. The rooted explants were transferred to greenhouse in peat moss and sand at various amounts 1:1, 1:2 and 2:1, respectively. The culture crowns were successfully disinfecting by Colorex 40% for 20 min with 77.77% survival and 100% free contamination. MS media at full strength was the best culture media showed shootlet number 1.75 shootlet/explant and shootlet length 2.25 cm with 9.25 leaf/shootlets. Among the different concentrations 2.0 mg/l BAP showed highest shoot proliferation of 56 and 42 shoots per explant at the first and second subculture, respectively. The longest shoot (5.5 and 5.5 cm) was produced in the two subcultures by the treatment combination of 0.25 mg/l BAP. The highest numbers of roots were produced by 1.0mg/l IAA were 10.67 roots/shootlet and the tallest length of roots were obtained for explants cultured on MS media containing IAA 3 mg/l. The individual rooted explants derived from plantlets were transferred to poly bags in the green house at mixture media after 7 days hardening in room temperature (28-30°C) and established plantlet was ready for planting.

Keywords:—Pineapple (Ananas comosus L. Smooth Cayenne), micropropagation, shoot proliferation, rooting stage, acclimatization