Alves et al. 2006 (Article) Vriesea

Micropropagation of the Brazilian endemic bromeliad Vriesea reitzii trough nodule clusters culture

Author(s):—G.M. Alves, L.L. Dal Vesco & M.P. Guerra

Publication:—Scientia Horticulturae 110(2): 204-207. (2006) — DOI

Abstract:—Vriesea reitzii Leme & Costa is an endemic bromeliad from the Atlantic Forest in South Brazil. The devastation of this biome threatens the extinction of this species that besides its role in the ecosystem has an ornamental value. Tissue culture techniques are important tools for the mass propagation of threatened bromeliad species. In the present work we established an in vitro regenerative protocol for the large-scale propagation, and improvement of this species. Young basal leaves used as explants showed 90.6% induction rate of nodule clusters in MS culture medium supplemented with 20.0μM 2,4-D and 1.0μM Kin. The subculture of these nodule clusters to MS medium with BAP, Kin and 2-iP resulted in a regeneration rate of 60shoots/g nodule clusters. Subsequent subculture to MS media supplemented with 2.5μM 2-iP and 0.5μM NAA and then to MS medium free of PGR enhanced the full development of plantlets. Plantlets longer than 3cm were successfully acclimatized showing a survival rate of 90%.

Keywords:—Bromeliads, , Tissue culture, Regeneration, Histology, Shoot-buds