Manhaes et al. 2020 (Article) Stigmatodon

Transferability of nuclear microsatellite markers to Stigmatodon species: a bromeliad genus endemic to vertical cliffs of inselbergs in Brazil

Author(s):—V.C. Manhães, D.R. Couto, F. Salgueiro & A.F. da Costa

Corresponding email:—manhaesvc@gmail.com

Publication:—Braz. J. Bot. 1-8. (2020) — DOI

Abstract:—Stigmatodon Leme, G.K.Br. & Barfuss is one of the most representative Bromeliaceae genera that grow on the steepest slopes of the inselbergs of southeastern Brazil. Inselberg environments are characterized as islands due to their restricted, disjunct distribution and geographical isolation. These features make these sites interesting models for evolutionary and population genetic studies. In this study we report, the cross-species transferability of nuclear microsatellite markers, originally developed for other Bromeliaceae, to four Stigmatodon species: Stigmatodon brassicoides, S. costae, S. goniorachis and Stigmatodon sp. nov. Twenty-four microsatellite markers were tested, and 12 of these were successfully amplified in the four studied species. In S. brassicoides and S. costae, 10 markers showed amplification with polymorphism, and in Stigmatodon sp. nov and S. goniorachis, all 12 markers showed polymorphisms. The number of alleles ranged from two to four in S. brassicoides; from two to three in S. costae; from two to six in Stigmatodon sp. nov; and from two to seven in S. goniorachis. HO values ranged from 0.05 (P2P19 locus) in S. brassicoides to 0.689 (VgA04 locus) in S. goniorachis. The set of amplified microsatellite markers described in this study will be a useful tool for addressing various evolutionary, ecological and conservation aspects of Stigmatodon species.

Keywords:—Atlantic Forest · Bromeliaceae · Cross-amplification · SSR · Tillandsioideae