Wöhrmann et al. 2012 (Article) Fosterella

Development of microsatellite markers in Fosterella rusbyi (Bromeliaceae) using 454 pyrosequencing

Author(s):—T. Wöhrmann, N. Wagner, F. Krapp, B. Huettel & K. Weising

Publication:—American Journal of Botany 99(4): e160-e163. (2012) — DOI

Abstract:—? Premise of the study: Polymorphic microsatellite markers were developed for Fosterella rusbyi (Bromeliaceae) to evaluate the population genetic structure and genetic diversity of natural populations of F. rusbyi and other Fosterella species in Bolivia. ? Methods and Results: 454 pyrosequencing technology was used to generate 73027 sequence reads from F. rusbyi DNA, which together contained 2796 perfect simple sequence repeats (SSRs). Primer pairs were designed for 30 loci, of which 15 were used to genotype 30 F. rusbyi plants from two geographical areas in Bolivia. All markers were polymorphic, with two to nine alleles in the overall sample. Cross-species amplification was tested in 10 additional Fosterella species. Seven loci showed consistent amplification in six or more species. ? Conclusions: The 15 SSR markers developed for F. rusbyi are promising candidates for population genetic analyses within F. rusbyi and other species of Fosterella.

Keywords:—Bromeliaceae, Fosterella rusbyi, marker polymorphism, microsatellites, pyrosequencing