Hmeljevski et al. 2013 (Article) Encholirium

Development of SSR Markers for Encholirium horridum (bromeliaceae) and Transferability to other Pitcairnioideae

Author(s):—K.V. Hmeljevski, M.B. Ciampi, C. Baldauf, M.S. Reis & R.C. Forzza

Publication:—Applications in Plant Sciences 1(4): 1200445. (2013) — DOI

Abstract:—Premise of the study: We developed a set of primers for Encholirium horridum , a species closely associated with inselbergs of the Atlantic Forest, to assess genetic diversity, genetic structure, and gene fl ow between populations of this species.
• Methods and Results: From an enriched genomic library, 10 primer pairs for polymorphic microsatellite regions were devel- oped. The average number of alleles ranged from eight to 20, and the observed and expected heterozygosities ranged from 0.000 to 1.000, and from 0.000 to 0.929, respectively, across the populations.
• Conclusions: These markers will be useful in evaluating genetic diversity, spatial genetic structure, analysis of gene fl ow by paternity, and characterization of mating system of E. horridum .

Keywords:—Atlantic Forest; Bromeliaceae; cross-amplifi cation; Encholirium horridum ; inselberg; microsatellites.