Margis 2015 (Conference Paper) Vriesea

Transcriptomic analyses of Vriesea carinata: diversity of cysteine proteinases and identification of microRNAs

Author(s):—R. Margis in Benko-Iseppon, A.M.; Alves, M. & Louzada, R. (2015) An overview and abstracts of the First World Congress on Bromeliaceae Evolution. Rodriguésia 66(2): A1-A66.

Publication:— (2015).

Abstract:—Small RNA and RNA-seq libraries were constructed to identify mcroiRNAs and their putative targets in Vriesea carinata, a native bromeliad species from the Brazilian Atlantic Rainforest. Illumina technology was used to perform high throughput sequencing and data was analyzed using bioinformatics tools. We obtained 2,191,509 mature miRNAs sequences representing 54 conserved families in plant species. Further analysis allowed the prediction of secondary structures for 19 conserved and 16 novel miRNAs. Potential
targets were predicted from pre-miRNAs by sequence homology and validated using RTqPCR approach.
This study provides the first identification of miRNAs and their potential targets of a bromeliad species. This
transcriptomic analysis also allowed the identification of 28 members of the papain/bromelain-like family
and more than a hundred other cysteine proteinases.

Keywords:—Next Generation Sequencing; Microrna; Cysteine Proteinase.