Carvalho et al. 2015 (Conference Paper) Vriesea

Optimization of in vitro seed germination of Vriesea minarum (Bromeliaceae), a vulnerable species from Serra da Piedade

Author(s):—Lucas Carvalho, Fátima de Cássia, Vera Lúcia dos Santos & Andréa Marques in Benko-Iseppon, A.M.; Alves, M. & Louzada, R. (2015) An overview and abstracts of the First World Congress on Bromeliaceae Evolution. Rodriguésia 66(2): A1-A66.

Publication:— (2015).

Abstract:—Vriesea minarum L. B. Smith is a rupicolous species and endemic to the Iron Quadrilateral region in Minas Geraisstate. It grows in places frequently disturbed by the process of extracting iron ore, which has had a large impacton their habitat. Its occurrence inside only a few protected areas of Campos Rupestres of the Serra da Piedade hasled to its categorisation as a vulnerable species. The aim of the work was to establish a protocol for in vitro seed germination that can be used to maintain genetic variability. Seeds were germinated and grown in water (T0) andMurashige and Skoog (MS) medium in the presence of light at 25°C. In vitro germinated seedlings were multiplied in MS medium supplemented with BAP (6-benzylaminopurine) and IAA (indole-3-acetic acid): control ? MS(T1); MS + 2.22 µM BAP (T2); MS + 0.57µM IAA (T3). The seed disinfection was effective for all treatments.The percentages of seed germination obtained were 60.1 % in water (T0) and 55.9 % in the MS medium (T1).After three months in MS medium, the largest shoot height and fresh matter were obtained in T3- IAA (115.3 ±2.1mm and 22.0 ± 0.6mg respectively), while statistical difference was not observed between the seedlings whichgrew in MS medium and water (T0). The concentration of pigments (total chlorophyll) was similar between T2and T3, but significantly higher in MS medium (T1 = 1.07 ± 0.32 mg g-1) and lower in water (T0 = 0.36 ± 0.09mg g-1). The results of these experiments demonstrated the viability of in vitro multiplication. When initiationof the culture is done through seeds obtained from a wide range of capsules, many different genotypes can beconserved. The development of micropropagation protocols from seeds is an alternative for conservation of thisspecies in in vitro germplasm banks.

Keywords:—Vriesea minarum; In Vitro Multiplication; Seed.