Wöhrmann et al. 2013 (Article) Dyckia

Development of 15 nuclear microsatellite markers in the genus Dyckia (Pitcairnioideae; Bromeliaceae) using 454 pyrosequencing

Author(s):—T. Wöhrmann, D.S. de Barros P., F. Krapp, A.M. Benko-Iseppon, B. Huettel & K. · Weising

Publication:—Conservation Genetics Resources 5: 81-84. (2013) — DOI

Abstract:—The genus Dyckia (Bromeliaceae) comprises 147 species that are distributed in Brazil and adjacent countries. Many species are rare and narrow endemics. We used 454 pyrosequencing to isolate 1,587 microsatellite loci in Dyckia marnier-lapostollei. Of 50 loci that were selected for primer design, 15 markers proved to be polymorphic in five populations from three heterologous species, Dyckia dissitiflora, Dyckia pernambucana and Dyckia limae. Numbers of alleles per locus varied from 3 to 30, and expected and observed heterozygosities ranged from 0.21 to 0.53 and from 0.16 to 0.44, respectively, in the overall sample. The 15 new microsatellite markers are promising tools for studying population genetics in Dyckia species.